Figure 3.

Camkk2 null hematopoietic stem cells have increased proliferation in vitro. KSL cells were sorted from WT and Camkk2 null mice and cultured with TPO, SCF and Flt-3L in the presence or absence of BM endothelial cells (TSF and ECs, respectively). Cell were harvested and analyzed on day 7. (a) Total cells number. (b and c) Absolute numbers of KL and KSL cells. (d) Cells recovered at day 7 were plated in methylcellulose media for colony formation and colonies (CFUs). Graphs report total CFUs normalized by total cell expansion. The experiment was replicated twice. Bars graph reports mean±S.E.M. *P<0.05, **P<0.01, ***P<0.005. **** P<0.001