Table 4.

NOTCH-associated pathway gene expression as assessed in the RNA-Seq analysis of cGVHD B cells after NOTCH2 blockade

Gene	↑ or ↓	log2 fold change	P	Adjusted P	Rank (of 12 880)
HES4	↓	−2.97	2.8 × 10−17	1.8 × 10−13	2
HES1	↓	−1.10	3.8 × 10−4	.062	80
LFNG	↑	0.660	.001	.105	133
HDAC2	↓	−0.541	.014	.321	543
MFNG	↓	−0.525	.016	.339	622
EP300	—	0.667	.061	.454	1 737
MAML2	—	0.552	.119	.510	2 997
NOTCH2	—	0.371	.165	.550	3 867
DTX2	—	0.300	.262	.622	5 430
HDAC1	—	−0.101	.595	.828	9 259
DTX1	—	0.030	.926	.976	12 209
DTX4	—	0.026	.933	.979	12 269

cGVHD B cells after NOTCH2 blockade from the experiment described in Table 2. P values and adjusted P values are indicated. The numerical rank for each gene of all 12 880 genes expressed above background is also indicated, based on the adjusted P value score.

—, Not significantly changed by NOTCH2 blockade.