Figure 2.

Effect of 5‐HT receptor blockade in WT mitral valves treated with NdF in vivo. Mitral valve architecture was determined in NdF‐treated mice in the presence of various 5‐HT receptor blockers. (A) Thickness, (B) endothelial cells and (C) interstitial cells numbers in WT mice treated for 28 days with NdF (1 mg·kg⁻¹·day⁻¹) (WT‐NdF), or NdF with sarpogrelate (2 mg·kg⁻¹·day⁻¹) (WT‐S), or NdF with SB204741 (1 mg·kg⁻¹·day⁻¹) (WT‐SB204), or NdF with SB206553 (1 mg·kg⁻¹·day⁻¹) (WT‐SB206), or NdF with ritanserin (2 mg·kg⁻¹·day⁻¹) (WT‐R), and NdF with pCPA (100 mg·kg⁻¹·day⁻¹) (WT‐P). Values are means ± SEM (n = number of animals per group). # P < 0.05, significantly different from WT‐C; * P < 0.05, significantly different from WT‐NdF; ANOVA and Dunnett's post hoc tests.