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. 2017 Sep 22;14(6):6776–6782. doi: 10.3892/ol.2017.7038

Figure 1.

Figure 1.

(A) Matrigel invasion assay of Sawano cells transfected with three sets of siRNA, A, B and C, LSR. Scale bar, 100 µm. The results are presented below as a histogram. Control vs. siRNA, **P<0.01. (B) Western blotting for LSR, TRIC, OCLN, CLDN-1, −3, −4 and −7 in Sawano cells transfected with three sets of siRNA against LSR. The results for the expression of LSR and CLDN-1 are presented as a histogram. Control vs. siRNA, *P<0.05, **P<0.01. (C) Immunostaining of LSR and CLDN-1 in Sawano cells transfected with siRNA-C against LSR. Scale bar, 20 µm. (D) Sp1 transcription factor activity in Sawano cells transfected with siRNA-C against LSR. NC vs. Sp1 control, **P<0.01; Sp1 control vs. Sp1 siRNA, ##P<0.01. siRNA, small interfering RNA; LSR, lipolysis-stimulated lipoprotein receptor; TRIC, tricellulin; OCLN, occludin; CLDN, claudin; NC, negative control.