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. 2017 Sep 21;14(6):6387–6394. doi: 10.3892/ol.2017.7010

Figure 2.

Figure 2.

Curcumol induces caspase-dependent cell death in MG-63 cells. (A) MG-63 cells were treated with 63.5 mg/l curcumol for the indicated time intervals and and analyzed by flow cytometry. (B) Apoptotic rate is shown in the histogram. Data are presented as the mean ± standard deviation from three independent experiments. (C) Cellular morphological changes in MG-63 cells treated with 63.5 mg/l curcumol for 0, 24, 48 and 72 h, respectively. (D) Densitometric analysis of percentage of cell death. (E) Cleaved caspase-3 protein levels were determined by western blot analysis in MG-63 cells incubated with 63.5 mg/l curcumol for 24, 48 and 72 h. GAPDH served as the loading control. (F) Densitometric analysis of cleaved caspase-3. (G) Proliferation inhibition effect of 63.5 mg/l curcumol on MG-63 cells with or without z-VAD-fmk. All data are presented as the mean ± standard deviation. The results (mean ± standard error) were from three independent experiments. #P<0.05 compared with the control group; *P<0.05 compared with the curcumol group alone. PI, propidium iodide; FITC, fluorescein isothiocyanate; CQ, chloroquine.