Figure 4.

Inhibition of autophagy by CQ suppressed curcumol-induced apoptosis. (A) Cells were pretreated with or without CQ for 1 h and then incubated with 63.5 mg/l curcumol for 48 h prior to cell viability evaluation by MTT assay. (B) Western blot analysis was performed with antibodies against LC3II, LC3I and cleaved caspase-3. GAPDH was used as the loading control. (C) Histograms showing LC3II/LC3I and cleaved caspase-3 protein levels. Data are expressed as the mean ± standard error of the mean of three independent experiments. ^#P<0.05 compared with the control group; *P<0.05 compared with curcumol treatment group. CQ, chloroquine; cur, curcumol; LC3, light chain 3.