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In vitro SHAPE analysis supports a rod-like conformation for the mc PSTVd (+)` RNA. (A) and (B) Results obtained with N-methylisatoic anhydride (NMIA) and 2-methylnicotinic acid imidazolide (NAI), respectively, coupled to computer-assisted prediction using RNAstructure. Nucleotides in red, yellow and black displayed high (more that 0.85), intermediate (0.85–0.40) and low (less than 0.40) SHAPE-reactivity. Other details regarding domains and loops as in the legend to Fig. 1.
