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Detection of the Mb:anti-Mb complex. (A) Mb:anti-Mb complexation is detectable in native-PAGE as a slight retardation of migration (lanes 4 and 5) compared to the free antibody (lane 3). (B) SEC-MS chromatograms showing the elution of myoglobin in the absence and presence of anti-Mb (solid line and dashed line, respectively). Ubiquitin was used as an internal standard.
