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Differences in deuterium uptake (%) between myoglobin-antibody complex and native myoglobin at different reaction times detected by TRHDX-MS. X-axis shows the sequence of the localized peptides. (A-C) Equilibrium workflow: Mb pre-incubated with anti-Mb for 30 minutes prior to HDX-MS analysis. (D-F) Kinetic workflow: Mb was introduced to anti-Mb antibody concomitantly with the onset of labeling in the TRESI mixer.
