Fig. 1.
Generation of MH-SCD163 and RAW264.7CD163 cell lines. MH-S and RAW264.7 cells were transduced with the indicated lentiviral constructs and puromycin-resistance cells were selected and subcloned. Each cell line was stained with anti-pCD163 mAb to measure pCD163 expression by immunofluorescence assay (a) and western blot (b). c Total RNA of each cell line was isolated and reverse transcribed to amplify the full-length gene coding for pCD163. GAPDH transcripts were amplified to normalize the total amount of input RNA. d The growth curves of cells transduced with the indicated lentivirus are shown. Cells for each clone were seeded at a concentration of 1×104 cells/well and split daily for eight consecutive days and half of the cells were counted to determine cell number. The average cell count for each clone at each time point was plotted against time. Values indicate the mean ± SD from three replicates