Figure 2.
Ablation of Caspase-8 does not prevent alcoholic liver injury in mice. WT (n=8–10) and Casp8Δhepa mice (n=7) were fed with isocaloric (ctrl) or Lieber-DeCarli (EtOH) diet for 8 weeks. (a) Serum AST and ALT activities after 8 weeks of feeding. (b) Representative H&E staining of liver sections from each treatment group. Arrows: ballooned enlarged hepatocytes with rarefied cytoplasm; Black asterisk: polymorphonuclear neutrophils; LD: lipid droplets. (c–e) Determination of pro-inflammatory effects through immunofluorescence staining for (c) CD45 (red, arrows), (d) CD11b (green, arrows) and (e) CD3 (red, arrows). Total cells are counterstained with DAPI (blue). ***P<0.001; *P<0.05; n.s.: not significant