Figure 1.

Generation of Ccnb1 conditional knockout mice. (a) The target strategy of Ccnb1. LoxP site were inserted behind exons 4 and 9 of Ccnb1 allele; when the floxed allele crossed with Cre, exons 5–9 were deleted. (b) Mating strategy to generate all germ cell conditional knockout Ccnb1 mice (Mvh-cKO). (c) Mating strategy to generate postnatal, premeiotic male germ cell conditional knockout Ccnb1 mice (Sta8-cKO). (d) Western blotting analysis of Ccnb1 in adult (8–12 weeks) Mvh-cKO and Control littermates’ testis. (e) Western blotting analysis of Ccnb1 in adult Stra8-cKO and Control littermates’ testis. (f) Real-time PCR analysis of Ccnb1 expression in adult Control and Mvh-cKO mice testis. (g) Real-time PCR analysis of Ccnb1 expression in adult Control and Stra8-cKO mice testis. In panels (f and g), ≥3 samples were used in each group in qPCR