Figure 3.

GBP2 is essential for IFN-γ-induced mitochondrial elongation and inhibition of breast cancer cell invasion. (a) IFN-γ-induced GBP2 in MDA-MB-231 cells was depleted by GBP2 shRNA but not scramble shRNA. A blot representative of three experiments is shown. (b) The histogram shows the mean GBP2 band density, which was measured and normalized to β-actin±S.E.M., n=3, *P<0.05. (c) GBP2 depletion abolished IFN-γ-induced inhibition of MDA-MB-231 cell invasion. Data shown are mean±S.E.M. (n=4), *P<0.05. (d) GBP1 in MDA-MB-231 was depleted by GBP1 shRNA but not scramble shRNA. A blot representative of three experiments is shown. (e) The histogram shows the mean GBP1 band density, which was measured and normalized to β-actin±S.E.M., n=3, *P<0.05. (f) GBP1 depletion had little effect on IFN-γ-induced inhibition of MDA-MB-231 cell invasion. Data shown are mean±S.E.M. (n=4). (g) Upper panel, the representative images of MDA-MB-231 cells transfected with the pSliencer-2.1 null vector or vector expressing GBP2 shRNA. Mitochondria were visualized with MitoTracker Red. Scale bar, 10 μm. Lower panel, data shown in the upper panel are the average mitochondrial lengths in 19–22 randomly selected MDA-MB-231 cells. Error bars, S.E.M., *P<0.05. (h) Upper panel, the representative images of MDA-MB-231 cells transfected with the pSliencer-2.1 null vector or vector expressing GBP1 shRNA. Scale bar, 10 μm. Lower panel, data shown in the upper panel are the average mitochondrial lengths in 19–22 randomly selected MDA-MB-231 cells. Error bars, S.E.M., *P<0.05