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. 2017 Sep 13;26(8):1341–1354. doi: 10.1177/0963689717720050

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© The Author(s) 2017

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Fig. 1. — Effects of cryopreservation using the 4 different freezing solutions on human hepatocytes viability and function (N = 6). (A) Cell viability immediately after thawing assessed by trypan blue exclusion, (B) cell viability assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, (C) cell attachment assessed by sulforhodamine B assay, (D) albumin production, and (E) urea synthesis after thawed and plated for 24 h. Statistical significance as compared to fresh hepatocytes (controls): *P < 0.05, **P < 0.01, and ***P < 0.001.