Table 1.
Cryopreservation Solution Mixtures.
| Main Solution | UW (ViaSpan®) | HTK (Custodiol™) | Bambankera | CryoStor CS10a |
|---|---|---|---|---|
| Application | Organ preservation solution | Organ preservation solution | Cell cryopreservation medium | Cell cryopreservation medium |
| Ingredients | K+-lactobionate (100 mM) KH2PO4 (25 mM) Na+ (30 mM) Raffinose (30 mM) Adenosine (5 mM) Glutathione (3 mM) Allopurinol (1 mM) Hydroxyethyl starch (50 g/L) Dexamethasone (8 mg/L) Penicillin (200,000 U/L) Insulin (40 U/L) Osmolarity 320 mOsM pH 7.4 | Na+ (15 mM) K+ (10 mM) Mg2+ (4 mM) Ca2+ (0.015) Cl− (50 mM) l-Histidine (198 mM) Ketoglutarate (1 mM) Tryptophan (2 mM) Mannitol (30 mM) Osmolarity 310 mOsM pH 7.2 | Ready to use Serum free DMSO (10%) | Ready to use Serum free DMSO (10%) [Modified from HTS] Composition of HTS: Na+ (100 mM) K+ (42.5 mM) Mg2+ (5 mM) Ca2+ (0.05 mM) Cl− (17.1 mM) H2PO4 − (10 mM) HCO3 − (5 mM) HEPES (25 mM) Lactobionate (100 mM) Sucrose (20mM) Mannitol (20mM) Glucose (5 mM) Dextran-40 (6%) Adenosine (2 mM) Glutathione (3 mM) Osmolarity 360 mOsM pH 7.6 |
| Additives | ||||
| DMSO | 10% | 10% | — | — |
| Glucose | 5% | 5% | — | — |
Note. UW, University of Wisconsin solution; HTK, histidine, tryptophan, ketoglutarate solution; HTS, hypothermosol; DMSO, dimethyl sulfoxide.
aThe actual composition of Bambanker and CryoStor CS10 are undisclosed.