Figure 5.

Human bone marrow–derived mesenchymal stem cells (hBM-MSCs) were incubated in chondrogenic medium without (control) and with GW0742, hyaluronic acid (HA), or Chondrogenic Hyaluronic Acid–Mesenchymal Stem Cells–PPAR-δ agonist (CHAMP) in 50% of human osteoarthritis (OA) synovial fluid for 14 d. The numbers of chondrocyte spheroid (>40 mm) were counted for bar graphs (A). The spheroids were stained with fluorescent agent (DiO) and collagen II were stained with fluorescein isothiocyanate (FITC) to confirm chondrocytes. The magnified image was taken using confocal microscope (B). On day 14, chondrocytes were processed for immunoblotting using antibodies to type II collagen, peroxisome proliferator–activated receptor (PPAR) δ, transforming growth factor (TGF) β (C–F), type I collagen, type X collagen, and β-actin (G–I). Protein band densities were measured to obtain bar graphs (C–I). Mean ± standard error, n = 3. *P < 0.05 versus control. **P < 0.01 versus control. Image is the representative of 3 independent experiments. Data presented are the result of 3 independent experiments. Scale bar: 80 μm.