Figure 3.

Hit determination of miRNA screen in C2C12 myoblasts. (A) Graphs represent the normalized ratio of all the readouts, calculated as described in the method section. The black line indicates the limit of miRNA inhibitors that induced a change superior to Negative control A ± 2σ_{Negative control A}, and the red line the limit of miRNA inhibitors that induced a change superior to Negative control A ± 3σ_{Negative control A}. (B) Graphs represent quantile–quantile (QQ) plots, plotting the theoretical quantiles for each assay over the normalized ratio. miRNA inhibitors were considered hits when they induced changes of at least A ± 2σ_{Negative control A} and deviated from the normal distribution. The gray points correspond to the compounds inducing a change inferior to Negative control A ± 2σ_{Negative control A}, the black points to a change comprised between the Negative control A ± 2σ_{Negative control A} and A ± 3σ_{Negative control A}, and the red points to a change superior to and A ± 3σ_{Negative control A}. (C) Hierarchical clustering of the 5 parameters for the 62 hits. miRNA inhibitors were considered hits when they induced changes of at least A ± 2σ_{Negative control A} and deviated from the normal distribution.