Figure 4.

Hit determination and hierarchical clustering of miRNA validation in C2C12 myotubes. (A) A subset of all readouts is depicted; graphs represent the normalized ratio of all the readouts, calculated as described in the methods section. The black line indicates the limit of miRNA inhibitors that induced a change superior to negative control A ± 2σ_{Negative control A}, and the red line the limit of miRNA inhibitors that induced a change superior to Negative control A ± 3σ_{Negative control A}. (B) Hierarchical clustering of the 5 parameters for the 44 hits. miRNA inhibitors were considered hits when they induced changes of at least A ± 2σ_{Negative control A}.