Glucose and lipid metabolism in mice with Brs3 deleted in Vglut2- or Vgat-expressing neurons and fed a high fat diet. (A–F) Brs3fl/y;Vglut2-Cre (Vglut2-Cre) and littermate control Brs3fl/y (flox/y) mice. (A–C) Glucose tolerance test, area under the curve (AUC), and insulin at indicated times after glucose challenge in mice at 17 weeks of age. (D, E) Insulin tolerance test and AUC at 19 weeks of age. (F) Serum triglyceride (TG) and cholesterol levels (N = 9/group). (G–L) Brs3fl/y;Vgat-Cre (Vgat-Cre) and littermate control Brs3fl/y (flox/y) mice. (G–I) Glucose tolerance test, area under the curve (AUC), and insulin at indicated times after glucose challenge. (J, K) Insulin tolerance test and AUC. (L) Serum triglyceride (TG) and cholesterol levels (N = 11–13/group). * indicates P < 0.05 by unpaired t-test.