Effect of Brs3 re-expression on body weight, composition, and energy homeostasis. Brs3 was re-expressed in Vglut2- or Vgat-expressing neurons in mice fed a chow or high fat diet (HFD). (A–E) Body weight, fat mass, lean mass, food intake, and energy expenditure on a high fat diet (HFD) of WT, Brs3-/y (KO), Brs3loxTB/y (loxTB), Brs3loxTB/y;Vglut2-Cre (Vglut2-Cre), and Brs3loxTB/y;Vgat-Cre (Vgat-Cre) mice (N = 5 WT, 5 KO, 19 loxTB, 10 Vglut2-Cre, 6 Vgat-Cre). (F–J) as above, on a chow diet (N = 8 WT, 8 KO, 16 loxTB, 10 Vglut2-Cre, 6 Vgat-Cre). P values were determined by 2-way RM ANOVA and are in Table S2.