Figure 4.

bFKB1 stimulates EE in adipose-selective Fgfr1 CKO mice on HFD. (A) Mean EE values in control WT and Fgfr1^Adipoq CKO mice receiving a single 10 mg/kg dose of indicated IgG (Arrow). Mice were maintained at thermoneutrality (30 °C) and were on HFD for 8 weeks at the time of dosing. (B) Mean daily EE value ± SEM in indirect calorimetry shown in (A) during the indicated pre- and post-dose periods. (C) Average % body weight change after 7 days of exposure to bFKB1. The pre-dose body weights were 45.1 ± 6.0 g (IgG) and 45.5 ± 4.5 g (bFKB1) for WT. For CKO mice, pre-dose weights were 44.9 ± 4.0 (IgG) and 44.8 ± 5.0 (bFKB1). (D) AUC in GTT performed on 9 days post-injection. (E) Serum parameters on day 11 after IgG or bFKB1 treatment. (F) Gene expression changes in BAT measured by qPCR. N = 6–7 mice per group. Gene expression normalized to IgG treated WT animals. In (A), error bars omitted for clarity. Other data are mean ± SEM. p < 0.05 (*), <0.01 (**), <0.001 (***) vs control.