Fig 5. Change in the exon-exclusion state of transcripts during expression of U6 snRNA in TDP-43-knocked down cells.

(A) Images of migrated bands of spliced forms of Dnajc5, Sort1, and Poldip3 transcripts. RPS18 was used as an internal loading control. Spliced forms of transcripts were distinguished using splicing-dependent pairs of PCR primers. (B) Relative amounts of the exon-excluded forms of Dnajc5, Sort1, and Poldip3 transcripts (mean ± SEM; n = 5). Significance was tested by Student’s t test: *p < 0.05, **p < 0.01, and ***p < 0.001. Numbers in each bar show mean values.