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Contour plot for size (A) with respect to cryoprotectant/phospholipids ratio and the number of homogenization steps (PEG proportion is 4.7; cholesterol concentration is 14.2 and freezing is set at −80 °C); and (B) with respect to cholesterol concentration and the number of homogenization steps (PEG proportion is 4.7; cryoprotectant/phospholipids ratio is 5 and freezing is set at −80 °C).
