Table 5.

Analysis of variance for prediction of lyo-LCL-SIM CQAs.

CQA	Source	Degrees of freedom	Sum of squares	Mean square	F value	P value
Liposomal size	Total corrected	20	1.6488	0.08244
	Regression	4	1.3252	0.3313	16.378	<0.001
	Residual	16	0.3236	0.0202
	Lack of fit	14	0.3184	0.0227	8.647	0.108
	Pure error	2	0.00526	0.00263
Liposomal SIM concentration	Total corrected	20	1,077,280	53863.80
	Regression	6	969,918	161,653	21.080	<0.001
	Residual	14	107,359	7668.47
	Lack of fit	12	93751.80	7812.65	1.148	0.557
	Pure error	2	13606.80	6803.40
ESR	Total corrected	19	0.1660	0.00874
	Regression	5	0.1255	0.0251	8.662	0.001
	Residual	14	0.0406	0.0029
	Lack of fit	12	0.0385	0.0032	3.056	0.273
	Pure error	2	0.00209	0.00105
Tm change	Total corrected	20	70.325	3.516
	Regression	5	59.393	11.878	16.299	<0.001
	Residual	15	10.931	0.729
	Lack of fit	13	9.524	0.733	1.041	0.592
	Pure error	2	1.407	0.704
Residual moisture content	Total corrected	19	24.958	1.314
	Regression	5	18.072	3.614	7.348	0.001
	Residual	14	6.887	0.492
	Lack of fit	12	6.076	0.506	1.250	0.528
	Pure error	2	0.810	0.405