MRL HFD mice do not have altered glucose oxidation, Glut2, Glut4 and pGlycogen SynthaseSer641/645 protein expression. (A) Western blot of liver lysates from B6 and MRL mice in CD and HFD conditions showing levels of Hexokinase II, Glut2, Glut4, and pGlycogen SynthaseSer641/645 with respective loading controls used for quantification. (B) Hexokinase II levels were unchanged in MRL, with a trend to increase in B6 HFD mice. (C) Glut2 is significantly lower in B6 HFD but it is not altered in MRL HFD. (D) Quantification shows a significantly lower basal Glut4 expression in the lysates of MRL CD, and expression level remains unaltered after a HFD. (E) pGlycogen SynthaseSer641/645 was decreased in B6 HFD mice, with no change observed in MRL mice. n=3 each; * p <0.05, ** p<0.01.