Table 1.
Activity of canonical pathways in hiPSC-vSMCs after stimulation with lactate or hypoxia
| Ingenuity Canonical Pathways | SMC-hypoxia | SMC-lac |
|---|---|---|
| EIF2 Signaling | −2.940 | −6.021 |
| HIPPO signaling | 2.496 | 3.051 |
| ILK Signaling | 2.111 | 3.015 |
| mTOR Signaling | 3.266 | 1.633 |
| p70S6K Signaling | 1.964 | 1.528 |
| CREB Signaling | 1.698 | 1.698 |
| AMPK Signaling | 2.000 | 1.000 |
| Calcium Signaling | 1.155 | 1.155 |
| PAK Signaling | 0.447 | 1.789 |
| Signaling by Rho family GTPases | 0.866 | 1.155 |
| Telomerase Signaling | 1.213 | 0.728 |
| Regulation of eIF4 and p70S6K Signaling | 0.943 | 0.943 |
| Ephrin B Signaling | 0.905 | 0.905 |
| Protein Kinase A Signaling | −0.745 | −1.044 |
| Insulin Receptor Signaling | −0.218 | −1.528 |
| GNRH Signaling | 0.853 | 0.853 |
| Rho A Signaling | −0.667 | −1.000 |
| JAK/Stat Signaling | 1.069 | −0.535 |
| Granzyme B Signaling | 0.378 | 1.134 |
| PTEN Signaling | −0.447 | −0.894 |
| Apoptosis Signaling | −1.091 | −0.218 |
| Acute Phase Response Signaling | 1.091 | −0.218 |
| CDK5 Signaling | −0.218 | −1.091 |
| Rac Signaling | −0.378 | −0.756 |
| VEGF Signaling | 0.000 | 1.069 |
| IGF-1 Signaling | 0.535 | 0.535 |
| Agrin Interactions at Neuromuscular Junction | 0.258 | 0.775 |
| PI3K/AKT Signaling | 0.343 | 0.686 |
| PDGF Signaling | −0.728 | −0.243 |
| ERK/MAPK Signaling | 0.000 | 0.354 |
Proteins in hiPSC-vSMCs that were quantitatively changed by L+ culture conditions or hypoxia were identified via proteomics analysis and then evaluated with Ingenuity Pathway Analysis software. The results for pathways that regulate cell proliferation, survival, and migration; protein synthesis; gene transcription; and synthetic SMC differentiation are displayed.