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. Author manuscript; available in PMC: 2017 Nov 13.
Published in final edited form as: Leukemia. 2017 May 10;31(12):2661–2669. doi: 10.1038/leu.2017.141

Figure 4.

Figure 4

KDM6B is upregulated by NF-κB signaling in MM.

(a) MM.1S cells were incubated with or without 30 ng/ml of TNFα and 20 μM of MLN120B or DMSO. After 5 min of treatment, whole cell lysates were extracted and subjected to immunoblot analysis with the indicated antibodies (left panel). After 2 h of treatment, total RNA and whole cell lysates were extracted and subjected to quantitative real time PCR and immunoblot analysis (right panel).

(b) MM cell lines were treated with conditioned medium from MM patient-derived bone marrow stromal cells (BMSC CM) or control culture medium for 2h.

(c) MM.1S cells were cultured with or without 20 μM of MLN120B in the presence of BMSC CM or control medium for 2h.

(a-c) Data represent the amount of mRNA relative to control. Error bars indicate s.d. of triplicate measurements. **P < 0.01, ***P < 0.001 compared with control.