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Structure of mycobacterial transposons utilized to generate random mutant libraries. Inverted repeat (IR) for Tn5367 or Tn5370 (filled triangle), or Himar1-derived transposon (striped triangle); tnpR, IS1096 resolvase; aph, kanamycin-resistant gene; tnpA, IS1096 transposase; res, resolution site for transposon gamma-delta; hyg, hygromycin resistant gene; and C9 Himar1, Himar1 transposase.
