Table 1.
Specificity of carbohydrates targeting natural antibodies in BALB/c mice.
| Glycan ID (#) | Structure | Common name | Median and MAD as RFU | Number of mice showing RFU ≥4,000 (%) | Number of human donors showing RFU ≥4,000 (%) |
||
|---|---|---|---|---|---|---|---|
| IgM | IgG | ||||||
| 060 | 6-O-Su-Galβ-spa | 61,113 | 1,156 | 100 | 73 | 0 | |
| 271 | Galβ1-6Galβ1-4Glcβ-sp | 53,622 | 1,934 | 100 | 55 | 27 | |
| 802 | Galβ1-3GalNAc(fur)β-sp | 51,348 | 2,324 | 100 | 73 | 9 | |
| 176 | 3-O-Su-Galβ1-4(6-O-Su)Glcβ-sp | 43,008 | 9,342 | 100 | 9 | 0 | |
| 166 | GlcAβ1-6Galβ-sp | 39,105 | 2,993 | 85 | 18 | 0 | |
| 150 | 3-O-Su-Galβ1-3GalNAcα-sp | 37,943 | 3,232 | 100 | 18 | 0 | |
| 437 | GalNAcα1-3(Fucα1-2)Galβ1-3GalNAcβ-sp | A(type 4) | 33,886 | 3,193 | 90 | 45 | 45 |
| 125 | 6-Bn-Galβ1-4GlcNAcβ-sp | 32,674 | 5,389 | 95 | 0 | 0 | |
| 154 | 3-O-Su-Galβ1-3GlcNAcβ-sp | 32,651 | 3,954 | 100 | 64 | 36 | |
| 177 | 3-O-Su-Galβ1-4(6-O-Su)GlcNAcβ-sp | 32,496 | 7,215 | 100 | 9 | 9 | |
| 287 | 3-O-Su-Galβ1-3(Fucα1-4)GlcNAcβ-sp | SuLea | 20,063 | 4,962 | 95 | 0 | 9 |
| 234 | Galβ1-4(Fucα1-3)GlcNAcβ-sp | Lex | 13,573 | 2,635 | 80 | 0 | 0 |
List of glycans with binding signals above 4,000 relative fluorescence units (RFU) in at least 80% of examined mice (n = 20). Comparison with human data (n = 11). The data obtained by Semiotik LLC, Russia, in the same conditions.
asp means aminoethyl, aminoprolyl, or glycyl spacer.