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. 2017 Nov 10;7:15282. doi: 10.1038/s41598-017-15543-x

Figure 2.

Figure 2

Effect of PCSK9 variants on LDL uptake in (A) transiently transfected HEK293 cells, (B) in HEK293 cells and (C) HepG2 cells incubated with purified PCSK9 variants. LDL uptake in transiently transfected HEK293 cells (A) was determined as described in Materials and Methods. For LDL uptake assay with purified PCSK9 variants, cells were incubated with the PCSK9 variants at 0.5 μg/mL during 2 h prior FITC-LDL addition (B and C). LDL internalization was determined after 4 h incubation at 37 °C as described in Materials and Methods. Values represent the mean ± standard deviation of 3 independent experiments performed by triplicate. *p < 0.01 versus no PCSK9 addition; #p < 0.01 versus wild-type (wt) PCSK9. p.(Asp374Tyr) GOF mutant was used as internal control.