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. 2017 Nov 10;7:15282. doi: 10.1038/s41598-017-15543-x

Figure 3.

Figure 3

Binding affinity curves of wild-type and PCSK9 variants for LDLR at both pH 7.2 and pH 5.5. Panel (A) wild-type (wt) PCSK9; panel (B) p.(Asp374Tyr); panel (C) p.(Pro331Ala); panel (D) p.(Arg357Cys); panel (E) p.(Ser636Arg) and panel F) p.(His643Arg). Solid-phase immunoassay for PCSK9-LDLR ectodomain binding was performed as described in Methods. Values represent the mean ± standard deviation of 3 independent experiments performed by triplicate.