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. 2017 Nov 10;7:15295. doi: 10.1038/s41598-017-15547-7

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(A) Brightfield image of non-treated OvCa429 MCA with (B) CARS image of −2930 cm⁻¹ has high CH₃ signal displayed in the (C) the maximum intensity pixel and average spectrum. Experimental parameters: Pump = 720 nm, 22.50 mW; Supercontinuum = 785–945 nm, 5.05 mW; Acquisition time = 500 ms/pixel; Map size = 51 × 51 pixels; Steps = 600 nm; Objective = 40x, 0.75NA. (D) Brightfield image of crystallized LPA in conditioned medium correlates well with the (E) CARS image of −2850 cm⁻¹ peak area. (F) The CARS spectra from the maximum intensity pixel and map average show three peaks at −2850, −2880, and −2930 cm⁻¹ from a combination of symmetric CH₂, asymmetric CH₂, and CH₃ stretches, respectively. Experimental parameters: Pump = 720 nm, 25.20 mW; Supercontinuum = 785–945 nm, 3.80 mW; Acquisition time = 500 ms/pixel; Map size = 41 × 41 pixels; Steps = 300 nm; Objective = 100x, 0.9 NA.