Figure 3.

Incremental increases in the allele number of members of the C. albicans MNN4-like gene family restores the ability to bind Alcian Blue in mutants with defects in the phosphomannosylation. Null mutant cells lacking either mnn4Δ (open bars) or mnt3Δ, mnt5Δ (closed bars) were transformed with expression vectors containing each of the members of the MNN4-like gene family, then were grown in YPD medium, and ability to bind Alcian Blue was measured. The strains used are: NGY152 (WT), CDH15 (mnn4Δ), NGY1227 (mnt3Δ, mnt5Δ), see Table 1 for details of other strains. The data represent the means ± SD of three independent assays performed by triplicate. ^*P < 0.05, compared with WT control cells. ^†P < 0.05 when compared to the null mutant strain. The open bars correspond to strains in which the mnn4Δ null mutant was complemented with MNN4 gene alleles. The closed bars refer to experiments in which the double mnt3Δ, mnt5Δ null mutant was complemented.