Figure 4.

Phagocytosis of C. albicans null mutants lacking members of the MNN4-like gene family by RAW 264.7 macrophages. C. albicans yeast cells were labeled with Acridine Orange and incubated with RAW 264.7 macrophages at a MOI 3:1 for 2.5 h at 37°C under CO₂. Macrophages were gated by FACS and 50,000 cells were counted/ sample. Results represent macrophages interacting with at least one fluorescent fungal cell. Strains used are: NGY152 (WT), CDH15 (mnn4Δ), HMY81 (mnn42Δ), HMY84 (mnn42Δ + MNN42), HMY198 (mnn41Δ, mnn42Δ, mnn47Δ), HMY199 (mnn41Δ, mnn42Δ, mnn47Δ + MNN41), HMY200 (mnn41Δ, mnn42Δ, mnn47Δ + MNN42), and HMY201 (mnn41Δ, mnn42Δ, mnn47Δ + MNN47). The data represent means ± SD for three independent assays performed by duplicate. ^*P < 0.05 when compared against the WT strain. ^**P < 0.05 when the octuple mutant was compared against the parental strain BWP17.