Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Nov 10;7:15285. doi: 10.1038/s41598-017-15325-5

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Metabolic Profiling of 2D vs. 3D cell culture model. (1A) Schematic of the microtissue perfusion insert placed in each well of a seahorse flux pack to enable localization of a spheroid during metabolic profiling. (B) Representative image (4X magnification) of an HCT116 spheroid within a microtissue perfusion insert. Average diameter 829.332 ± 27.13 µM. (C) OCR of HCT116 spheroids over time plotted individually (n = 16). (D) Oxygen Consumption Rate (OCR) of the spheroids as a group, OCR is maintained over a period of 140 mins. (E) Mitochondrial stress test in an HCT116 colorectal cancer 2D monolayer 12.5k cells per well, undertaken using Seahorse XFe96 Extracellular Flux Analyzer. Oligomycin (1 uM), FCCP(1 uM) and Rotenone/Antimycin A(1 uM) used to test mitochondrial metabolism. (F) Mitochondrial stress test in an HCT116 colorectal cancer 3D spheroid model. G: Coefficient of variation <20% within the spheroid OCR values observed. Error bars represent + -SEM.