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. 2017 Nov 10;7:15285. doi: 10.1038/s41598-017-15325-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Microtissue Viability Correlates to Metabolic Phenotype. (A) Oxygen Consumption Rate (OCR) of three individual HCT116 derived tumor microtissues. (B) ECAR of three individual HCT116 derived tumor microtissues. (C–E) Hematoxylin and Eosin staining of tumor microtissue slices. (F) Digital Quantification of necrotic regions. (G) Positive Correlation of percent viable tissue from H and E staining with metabolic oxygen consumption rate, n = 6.