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. 2017 Nov 10;8:1428. doi: 10.1038/s41467-017-01425-3

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 10 — KSRP and miR-129 regulate monocytic and granulocytic differentiation in vivo. a A schematic representation of the in vivo experimental design for the transplantation of CD34⁺ HPCs isolated from cord blood into mice (n = 4). b The CD14⁺ and CD11b⁺ populations in BM from mice transplanted with lenti-sh-KSRP or lenti-GFP-transduced HPCs were monitored by FACS. c qPCR analysis of pri-miR-129 and mature miR-129 expression in BM from mice transplanted with the lenti-GFP- or lenti-129-transduced HPCs. d The CD14⁺ and CD11b⁺ populations in BM from mice transplanted with lenti-129- or lenti-GFP-transduced HPCs were monitored by FACS. The quantitation is shown in the left panel. e Schematic representation of a model describing the role of the KSRP-miR-129-RUNX1 axis in regulating the normal lineage-specific differentiation of CD34⁺ HPCs. f qPCR analysis of pri-miR-129 and mature miR-129 expression in BM from mice transplanted with lenti-GFP- or lenti-sh-KSRP-transduced CD34 + HPCs. g Immunoblot of RUNX1 expression in BM from mice transplanted with lenti-129- or lenti-GFP-transduced HPCs. h Immunoblot of KSRP and RUNX1 expression in BM from mice transplanted with lenti-sh-KSRP- or lenti-GFP-transduced HPCs. Data are shown as means±s.d. *P < 0.05, **P < 0.01, ***P < 0.001, Student’s t-test