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. 2017 Nov 10;8:1428. doi: 10.1038/s41467-017-01425-3

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — KSRP regulates primary miRNA processing in myeloid cells. a Upper: schematic representation of two miR-129 loci in the human genome; lower: qPCR analysis of the relative abundance of the two pri-129 transcripts in THP-1 and NB4 cells. The expression of each transcript was normalized to pri-129-2 using the ΔΔCq method. Three technical replicates from a single experiment representative of two independent experiments. b Northern blot analysis of miR-129 transcripts in THP-1 cells during monocyte differentiation and NB4 cells during granulocyte differentiation. The upper panels show the hybridization of pri-129-1 with digoxigenin (DIG)-labeled RNA probes; rRNA was used as a loading control. The lower panels show the hybridization of pre-miR-129-1 and miR-129 with isotope-labeled DNA probes; U6 snRNA was used as a loading control. Relative expression of pri-, pre-, and mature miR-129 was quantified using Image J software. c qPCR analysis of the expression of pri-129-1, pre-miR-129-1 and miR-129 in the CD34⁺ HPC models of monocytic and granulocytic differentiation. d qPCR analysis of the expression of miR-129 in the monocytic and granulocytic differentiation of bone marrow derived CD34⁺ HPCs. e Left: qPCR of the expression of pri-129-1, pre-miR-129-1 and miR-129 in THP-1 cells transfected with pSIH-sh-KSRP or pSIH control. Right: qPCR of the expression of pri-129-1, pre-miR-129-1 and miR-129 in THP-1 cells transfected with pEGFP-KSRP or the control. Three technical replicates from a single experiment. Data are shown as means±s.d. **P < 0.01, ***P < 0.001, Student’s t-test