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. 2017 Nov 10;7:15297. doi: 10.1038/s41598-017-15717-7

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Characterization of EVs in unfiltered conditioned media, UF-SEC EVs and UC EVs. (a) Size distribution histogram of the EVs in (a) unfiltered conditioned media (b) UF-SEC EV isolates and (c) UC EV isolates as determined by TRPS. Graph shows the mean ± SEM, n = 5. (d) The percentage of particles >200 nm in conditioned media, UF-SEC EVs and UC EVs according to TRPS. The bar indicates the median diameter. **p 0.001–0.01 (e) Size distribution of EVs in UF-SEC and UC isolates based on cryo-TEM. The bar indicates the median diameter. (f) Low magnification and (g) detail cryo-TEM recording of UF-SEC isolated EVs from BEAS-2B.