Fig. 1.

Paravascular localization of P40D680 on the pial surface and in the cortex. a–c Images of paravascular P40D680 on the ventral, lateral, and dorsal surfaces of the brain. Mice were euthanized 10 min after the completion of intraventricular infusion of P40D680. Images are representative of n = 5 mice. Scale bars: 1 mm. MCA: middle cerebral artery. C of W: Circle of Willis. d–f Images of paravascular P40D680 at t = 10 min, 30 min, and 60 min after infusion. Representative of n = 5 mice of each group. Scale bars: 2 mm. g Representative detailed image of the dorsal surface of the brain showing paravascular localization of P40D680 at t = 60 min. GFP label represents autofluorescence channel. Scale bars: 200 µm. h Representative GFP and P40D680 overlay image of penetrating arteries and paravascular localization of P40D680 at t = 60 min. Scale bar: 200 µm