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. 2017 Nov 10;8:1434. doi: 10.1038/s41467-017-01484-6

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Tracer outflow to draining lymph nodes in the neck. a Representative pictures of tracer within the left deep cervical lymph node and afferent and efferent lymphatic vessels at euthanization at 10 min after P40D680 infusion into the right lateral ventricle of a Prox1-GFP mouse. Scale bars: 1 mm. b Quantification of fluorescent signal of deep cervical lymph nodes at t = 10 min, 30 min, and 60 min after infusion. n = 5 mice each time point. Red dashed line indicates baseline values from uninjected mice. Data are the mean ± SD. *p < 0.05 (one-way ANOVA with the Tukey’s multiple comparison). c Representative pictures of tracer within the mandibular lymph nodes and afferent and efferent lymphatic vessels at euthanization at 30 min after P40D680 infusion into the right lateral ventricle of a Prox1-GFP mouse. Scale bar: 1 mm. d Quantification of fluorescent signal of mandibular lymph nodes at t = 10 min, 30 min, and 60 min after infusion. n = 5 mice each time point. Red dashed line indicates baseline values from uninjected mice. Data are the mean ± SD. **p < 0.01 (one-way ANOVA with the Tukey’s multiple comparison)