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. 2017 Nov 10;7:15339. doi: 10.1038/s41598-017-15582-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Excess heat capacity profiles Δc_p (solid lines) and volume expansion coefficients α_V (open circles) for DMPC MLVs in the presence of 0 mol%, 13 mol%, 25 mol% and 50 mol% cholesterol (from bottom to top). For 50 mol%, the melting profile was so much broadened that no heat capacity anomaly could be identified. The volume expansion coefficient does not display an anomaly, either. The α_V values were corrected for the baseline of pure water but plotted without subtracting the value of the volume expansion outside of the phase transition.