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. 2017 Nov 10;7:15294. doi: 10.1038/s41598-017-14813-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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PKM2 mRNA, protein expression and enzyme activity in HCC. (A) The expression levels of PKM2 were measured with quantitative real-time PCR in 94 NCL-HCC and CL-HCC tissues. (B) The expression levels of PKM2 in the 125 HCC tumour and adjacent non-tumour liver tissues (HCC groups consist of randomly selected NCL-HCC and CL-HCC). (C) Protein levels of PKM2 in 9 representative HCC tissues (T) and adjacent non-tumour liver tissues (N) were analysed by western blotting. The uncropped blots details are provided in Supplementary Fig. S1. The relationship between PKM2 and GAPDH is provided in Supplementary Fig. S2. (D) Pyruvate kinase activity in HCC, CL-HCC and NCL-HCC, *P < 0.05.