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. 2017 Nov 10;7:15268. doi: 10.1038/s41598-017-15311-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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L1-80 levels are decreased in reeler mice. (a) Immunoblot analysis of homogenates from cerebellum (cere), cerebral cortex (cortex) and hippocampus (hippo) of 6-day-old wild-type (WT) and reeler mice with an antibody against the intracellular L1domain (L1-ICD). L1-FL: full-length L1. (b) Quantification of L1-FL and L1-80 levels in homogenates from cerebellum, cerebral cortex and hippocampus of wild-type and reeler mice. Mean values + SEM from 6 independent experiments and differences between groups are shown (*p < 0.05, **p < 0.01, ***p < 0.005; two-tailed t-test). RI: relative intensity in arbitrary units (AU). (c) Unaltered NCAM and CHL1 expression levels in cerebellar homogenates from wild-type (WT) and reeler mice. (d) Unaltered L1-80 levels in cerebellar and cerebral cortex homogenates from wild-type (Dab1 ^+/+), heterozygous (Dab1 ^+/−), and Dab1 knock-out (Dab1 ^−/−) mice, and in homogenates from ApoER2-VLDLR double knock-out (A^−/−V^−/−), heterozygous (A^+/+V^−/−, A^−/−V^+/+) and wild-type (A^+/+V^+/+) littermates. (a,c,d) Representative immunoblots out of 6 independent experiments are shown and display all L1, CHL1 and NCAM forms. GAPDH antibody was used to control loading and only the regions of the blots with GAPDH bands are shown.