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. 2017 Nov 10;7:15280. doi: 10.1038/s41598-017-15527-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Differences of phenotypic marker expressions on the Tregs in chronic hepatitis B patients and healthy individuals. (a) Histograms represented the frequency of CCR4, CCR5, CCR7, Ki67, and Tim-3 expression in Tregs. One representative experiment of gating on CD4⁺FoxP3⁺ population was shown. The isotype-matched antibodies were used as controls. (Gray area represents isotype-matched antibody, blue line represents HBV patient, and red line represents healthy control). (b) Frequency of Helios expressing Tregs of chronic HBV-infected patients in inflammatory phases and healthy individuals. (c) The proportion of CCR4, CCR5, CCR7, Ki67, and Tim-3-expressing Tregs within the CD4⁺FoxP3⁺T cells was shown. Error bars denote the mean ± standard deviation are presented. *p < 0.05, **p < 0.01, ***p < 0.001.