Figure 2.

Analysis of NPRL2 proteome. (a) Affinity purification of GFP, FLAG-NPRL2-GFP and NPRL3–GFP from corresponding HEK293 cells stably expressing these proteins was performed as described in Methods. Co-precipitating proteins were resolved by SDS-PAGE and visualized by Coomassie blue staining. (b) Heat map comparing proteins co-precipitating with GFP, FLAG-NPRL2-GFP and NPRL3–GFP. Shown are color-coded relative protein abundances (see Methods). (c,d) Classification of proteins co-purified with NPRL2 according to their localization (c) and biological process (d) was done by Gene Ontology Database. (d) Protein interaction networks of GATOR1 complex. The interaction networks between the GATOR1 complex and selected proteins from NPRL2 interactome known to be involved in the processes of DNA damage response (Supplementary Table S3) was done using protein interaction information from the STRING database (https://string-db.org/).