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. 2017 Nov 10;7:15262. doi: 10.1038/s41598-017-15509-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Amniotic membrane (AM) promotes cell protrusion generation and Paxillin expression in migrating Mv1Lu cells. (a) Detailed pictures of the migrating edge of artificial wound assays treated with AM in combination with inhibitors. Scale Bar 50 µm. (b) Western Blot of total protein extracts from sub-confluent Mv1Lu cells cultured in the presence of AM and/or inhibitors and collected after 24 hours. The dashed grey lines indicate that two distant parts of the very same blot were put together. ß-actin was used as loading control. (‡) Unspecific bands. (c) Relative protein level plots generated from Western Blot quantification. C: serum starvation; JNKi: SP600125; MEKi: PD98059. Asterisks denote statistically significant differences between conditions according to ANOVA statistical analysis: (***) p < 0.001; (ns) not significant. All experiments were repeated at least three times. Representative pictures and results are shown.