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. 2017 Nov 10;7:15262. doi: 10.1038/s41598-017-15509-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Amniotic membrane (AM) treatment increases the detection focal structures in migrating HaCaT cells. (a) Confocal microscopy images of HaCaT cells at the migrating edge of artificial wound assays. Scale Bar 50 µm. (b) 2.5x magnified detail from merged pictures in (a). (c) Plots for average number and size of focal structures detected at the migrating leading edge. Asterisks denote statistically significant differences between conditions according to ANOVA statistical analysis: (*) p < 0.05, (**) p < 0.005 and (***) p < 0.001; (ns) not significant. Paxillin: green; Actin: red; Nuclei: blue; C: serum starvation; JNKi: SP600125; MEKi: PD98059; FS: focal structures. All the experiments were repeated at least three times. Representative pictures are shown.