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. 2017 Nov 10;7:15240. doi: 10.1038/s41598-017-15695-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Number of Rim1/2 and Neurexin-1α clusters detected with different clustering algorithms. (a) Example for a Rim1/2 decorated IN targeting AZ from the molecular layer of the CB. Panels show the cluster-assignment of gold particles by DB, AP, MS and BC algorithm (different colors represent different clusters, black illustrates noise). (b) Number of Rim1/2 clusters detected by the different clustering algorithms. Open circles indicate individual AZs (n = 22 AZs), red symbols represent the mean ± SD. The black filled circles correspond to the AZ shown in a. (c,d) Same as in a and b, respectively, but for Neurexin-1α (n = 37 individual AZs).