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. 2017 Nov 6;11:599. doi: 10.3389/fnins.2017.00599

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Copyright © 2017 Colgan, Ganeshan, Harrison, Ismail, Holmes, Wells, Powell, O'Callaghan, O'Neill, Murray, Ahmed, Collins, Johnson, Groves and Lythgoe.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Immunohistochemistry to estimate regional PG-5 positive NFT density. (A) Single slice from a representative rTg4510 mouse with staining for PG-5 positive NFTs. Marked regional dependence of NFT density is observable [see inset (B–D) for examples of cortical (B), hippocampal (C), and thalamic (D) NFT distribution]. (E) Quantitative regional estimates of NFT density for each of the 16 WT and 9 TG mice that underwent MRI (8.5 month age).